Medium data
Search for medium no. = [417].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
417 MJYTFSH MEDIUM
| NaCl | 20.0 | g |
| K2HPO4 | 0.9 | g |
| KH2PO4 | 0.7 | g |
| CaCl2·2H2O | 1.0 | g |
| NH4Cl | 1.25 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 5.0 | mg |
| Na2SeO3·5H2O | 5.0 | mg |
| Fe2(SO4)3·xH2O | 0.01 | g |
| H2WO4 | 5.0 | mg |
| FeSO4·7H2O | 0.02 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Tryptone (BD-Difco) | 1.0 | g |
| Sodium formate | 0.2 | g |
| Trace mineral solution (see Medium No. 369) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
| Sulfur (powder) | 10.0 | g |
| Na2S·9H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Mix ingredients, except Trace vitamins, NaHCO3 and sulfur, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S·9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sterilized sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, sparge for 5 min with the same gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add the sterilized Na2S·9H2O solution to the medium, and pressurize to 200 kPa H2-CO2 (4:1, v/v). Readjust pH to 5.5, if necessary.
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