Medium data

Search for medium no. = [387].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

387 PYGV AGAR

Peptone	`0.25`	g
Yeast extract	`0.25`	g
Mineral salt solution (see Medium No. 304)	`20.0`	ml
2.5% Glucose solution	`10.0`	ml
Vitamin solution (see Medium No. 304)	`10.0`	ml
Agar	`15.0`	g
Distilled water	`960.0`	ml

Add components, except Glucose solution and Vitamin solution, to distilled water and mix thoroughly. Gently heat and bring to a boil. Autoclave and then cool to 45 - 50C. Aseptically add the filter-sterilized Glucose solution and Vitamin solution. Adjust pH to 7.5 with sterile KOH, if necessary.