355 METHANOSPHAERA MEDIUM
Rumen fluid, clarified (see below) | 100.0 | ml |
Yeast extract (BD-Difco) | 2.0 | g |
Trypticase peptone (BD-BBL) | 2.0 | g |
Sodium acetate | 0.5 | g |
Sodium formate | 0.5 | g |
Trace minerals (see Medium No. 151) | 10.0 | ml |
Trace vitamins (see Medium No. 197) | 1.0 | ml |
Na2SeO4 | 0.19 | mg |
NiCl2·6H2O | 0.7 | mg |
FeSO4·7H2O | 3.0 | mg |
KH2PO4 | 2.8 | g |
K2HPO4 | 0.6 | g |
(NH4)2SO4 | 0.3 | g |
NH4Cl | 1.0 | g |
NaCl | 0.6 | g |
MgSO4·7H2O | 0.15 | g |
CaCl2·2H2O | 0.076 | g |
Methanol | 5.0 | ml |
Resazurin | 1.0 | mg |
L-Cysteine·HCl·H2O | 0.875 | g |
Na2S·9H2O | 0.375 | g |
Distilled water | 900.0 | ml |