Medium data

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Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

30   POTATO-DEXTROSE AGAR

Potato, peeled and cut  200.0     g
Glucose  10.0     g
Agar  15.0     g
Distilled water  1.0     L
Boil potatoes for 20 min. Strain off the pieces of potatoes through a fine sieve. Add glucose and agar, and boil until dissolved. Do not use new potatoes.
Adjust pH to 5.4-5.6.
Comment: Commercial Potato dextrose agar (BD-Difco or Oxoid) is also available.