Medium data

Search for medium no. = [264].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

264   T2U MEDIUM

Polypepton (Nihon Pharm. Co.)  10.0     g
Beef extract (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  2.0     g
Glucose  10.0     g
Uracil  100.0     mg
Agar  15.0     g
Distilled water  1.0     L
Adjust pH to 7.0-7.2 with NaOH.
Aseptically add 1 ml of erythromycin solution (10 mg/ml 50% ethanol) to the medium autoclaved.



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