Medium data
Search for medium no. = [180].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
180 THERMOPLASMA MEDIUM
| (NH4)2SO4 | 0.2 | g |
| KH2PO4 | 3.0 | g |
| MgSO4·7H2O | 0.5 | g |
| CaCl2·2H2O | 0.25 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Glucose | 10.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 2.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium.
Comment: For JCM 13583, adjust pH to 3.0.
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