Medium data
Search for medium no. = [170].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
170 ARTIFICIAL DEEP LAKE VITAMIN MEDIUM
NaCl | 180.0 | g |
MgCl2·6H2O | 75.0 | g |
MgSO4·7H2O | 7.4 | g |
KCl | 7.4 | g |
CaCl2·2H2O | 1.0 | g |
Sodium succinate | 10.0 | g |
Yeast extract (BD-Difco) | 1.0 | g |
Vitamin solution (see below) | 10.0 | ml |
Add components, except vitamin solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.4. For preparation of solid medium, add 20.0 g/L Noble Agar (BD-Difco). Autoclave. After cooling to 50C, aseptically add the following vitamin solution (filter-sterilized):
Vitamin solution:
Biotin | 0.1 | g |
Vitamin B12 | 0.1 | g |
Thiamine·HCl | 0.1 | g |
Distilled water | 1.0 | L |
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