Medium data

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14   EG MEDIUM

`Lab-Lemco' powder (Oxoid)  2.4     g
Proteose peptone No. 3 (Difco)  10.0     g
Yeast extract (Difco)  5.0     g
Na2HPO4  4.0     g
Glucose  1.5     g
Soluble starch  0.5     g
L-Cystine  0.2     g
Agar  15.0     g
L-Cysteine·HCl·H2O  0.5     g
Horse blood  50.0     ml
Distilled water  1.0     L
Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6 - 7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.