Medium data

Search for medium no. = [1196].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1196   ACIDIHALOBACTER MEDIUM

Solution A:
NaCl  15.0     g
(NH4)2SO4  0.2     g
MgSO4·7H2O  0.4     g
K2HPO4  0.1     g
Distilled water  900.0     ml
Adjust pH 1.7 with H2SO4 and autoclave.
Solution B:
FeSO4·7H2O  13.9     g
Distilled water  100.0     ml
Adjust pH to 1.25 and autoclave at 115C for 10 min or filter-sterilize.
Solution C:
K2S4O6  0.3     g
Distilled water  10.0     ml
Sterilize by filtration.
Solution D:
Yeast extract  0.1     g
Distilled water  10.0      ml
Sterilize by autoclaving.
Aseptically combine solutions A and B after cooling. Then, add solutions C and D to the medium.



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