Medium data

Search for medium no. = [1158].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1158   GHIORSEA BIVORA MEDIUM

Artificial saltwater (see Medium No. 629)  1.0     L
Wolfe's mineral solution (see Medium No. 265)  1.0     ml
NaHCO3  1.68    g
Mix components thoroughly. Gas with N2-CO2 (4:1, v/v) to establish pH 6.5. Dispense the medium in culture vessels (e.g., 20 ml in 30 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling add per liter the following solution (filter-sterilized):
Modified trace vitamins (see below)  1.0     ml
Prior to inoculation, add filter-sterilized 5% H2 and 5% air to the gas phase (by volume). Incubate statically. Replenish H2 and air every two days.
Modified trace vitamins:
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl  5.0     mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
DL-Calcium pantothenate  5.0     mg
Vitamin B12  0.1     mg
p-Aminobenzoic acid  5.0     mg
Lipoic acid  5.0     mg
KH2PO4  0.9     g
Distilled water  1.0     L



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