Medium data

Search for medium no. = [1013].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1013 ACETIVIBRIO MEDIUM

Basal medium (see below)	`950.0`	ml
Trace element solution SL-10 (see Medium No. 433)	`1.0`	ml
Selenite-tungstate solution(see Medium No. 554)	`1.0`	ml
Vitamin solution (see Medium No. 197)	`10.0`	ml
NaHCO₃ (5%, w/v)	`30.0`	ml
Sucrose (20%, w/v)	`10.0`	ml
Yeast extract	`50.0`	mg
Na₂S·9H₂O	`0.25`	g

Autoclave Basal medium supplemented with Trace element solution SL-10 and Selenite-tungstate solution under 100% N₂ gas. Separately autoclave sucrose and yeast extract, and add to the medium. Filter-sterilize Vitamin solution and NaHCO₃ solution, and add to the medium. Distribute the medium into culture vessels under 100% N₂ gas and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na₂S·9H₂O solution (autoclaved under N₂ gas). Readjust pH to 7.7 - 7.9, if necessary.
Basal medium:

KH₂PO₄	`1.4`	g
NH₄Cl	`0.5`	g
MgCl₂·6H₂O	`0.2`	g
CaCl₂·2H₂O	`0.15`	g
Resazurin	`1.0`	mg
Distilled water	`950.0`	ml