Medium data
Search for medium no. = [1002].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1002 YTPS MEDIUM
| Yeast extract (BD-Difco) | 5.0 | g |
| Tryptone (BD-Difco) | 5.0 | g |
| Sodium pyruvate | 3.0 | g |
| NaCl | 19.0 | g |
| MgCl2·6H2O | 9.0 | g |
| MgSO4·7H2O | 0.15 | g |
| CaCl2·2H2O | 0.3 | g |
| KCl | 0.5 | g |
| KH2PO4 | 0.42 | g |
| (NH4)2SO4 | 0.1 | g |
| NaBr | 0.05 | g |
| SrCl2·6H2O | 0.02 | g |
| Na2SeO4 | 0.02 | g |
| Wolfe's modified mineral elixir(see Medium No. 221) | 5.0 | ml |
| Vitamin solution (see Medium No. 221) | 0.5 | ml |
| PIPES | 6.05 | g |
| L-cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 7.3.
Mix ingredients, except L-cysteine·HCl·H2O, and bring to the boil while gassing with N2-H2 (95:5) gas mixture. When the color turns from blue to reddish pink, add L-cysteine·HCl·H2O during continuous gassing and boiling. Anaerobically dispense the medium in tubes under a N2-H2 (95:5) atmosphere. Stopper the tubes and autoclave.
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