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| Keep the ampoule on a flat place, and make a file-cut at the neck. |
Wipe the ampoule with cotton-wool containing 70% alcohol. |
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| Cover the ampoule with a sterile cotton sheet, and cut it carefully at the neck. Do not use a cotton sheet containing alcohol. |
Using a sterile Pasteur pipette, add 0.3 to 0.5 ml of suitable rehydration fluid into the ampoule. |
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| Spread the sample on a suitable plate and incubate it under the directed condition. The subculture should be established in fresh media only after confirming its purity. A careless single-colony isolation may lead to picking up an unusual strain. It is recommended to revive the strain simultaneously using liquid culture. |
How to Revive Lyophilized or L-Dried Cultures (PDF 320KB) |
